Coding

Part:BBa_K106692:Design

Designed by: Andrew Horwitz   Group: iGEM08_UCSF   (2008-10-28)


Sir3, AarI A!D part (A!D variant!)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1313
    Illegal EcoRI site found at 2911
    Illegal PstI site found at 752
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1313
    Illegal EcoRI site found at 2911
    Illegal PstI site found at 752
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1313
    Illegal EcoRI site found at 2911
    Illegal BglII site found at 321
    Illegal BglII site found at 694
    Illegal BglII site found at 916
    Illegal XhoI site found at 2833
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1313
    Illegal EcoRI site found at 2911
    Illegal PstI site found at 752
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1313
    Illegal EcoRI site found at 2911
    Illegal PstI site found at 752
    Illegal AgeI site found at 1439
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2650
    Illegal SapI site found at 1550


Design Notes

Sir3 does not tolerate amino-terminal fusions, so we have redesigned the AarI A site. The new site is termed A!, introduces no additional amino acids at the N-terminus, and only works for cloning into an A! acceptor vector. For more information, please see the UCSF 2008 iGEM wiki.


Source

Sir3 was PCR amplified from yeast genomic DNA.

References